Name：GUO Zhengyan

Department：Department of Microbial Metabolic Engineering

Tel: 15711392599

Email: guozhengyan@imb.cams.cn; guozhengyan@imb.pumc.edu.cn

Education & Research Experience :

2021/09 – Now    Professor, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

2017/09–2021/08  Associate Professor, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

2014/09–2017/08  Assistant Professor, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

2012/10–2014/08 Postdoctoral researcher, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

2010/06–2014/08 Lecturer, School of Resources and Environment, Anhui Agricultural University, Hefei, China

2006/09–2009/07  Ph.D. in Northwest A &F University, Yangling, China

2003/09–2006/07 M.S. in Northwest A &F University, Yangling, China

1999/09–2003/07  B.S. in Northwest A &F University, Yangling, China

Research Field：

Discovery and biosynthesis of microbial natural products for anti-infective and antitumor therapies

Research Interests：

Dr. Guo has been engaged in discovery, biosynthesis and synthetic biology of bioactive molecules for anti-infective and antitumor therapies from microorganisms, such as actinomyces. Briefly, based on bioinformatics, molecular genetics, natural products chemistry and biochemistry technical methods, his research focus on genome-driven mining for microbial secondary metabolites with anti-infective or antitumor activities efficiently, biosynthetic mechanism of some important microbial natural products, which will expend the source of drug molecules and provide the guarantees for the sustainable development of new drugs.

Selected Publications :

1. Ma, Y.;# Guo, Z.;# Cao, B.;# Zhang, Y.;# Liu, X.; Yu, Y.; Tang, N.; Tong, X.; Wang, M.; Ye, X.; Feng, J.; Chen, Y.*; Wang, J.* Identification of antimicrobial peptides from the human gut microbiome using deep learning. Nat Biotechnol 2022, https://doi.org/10.1038/s41587-022- 01226-0

2. Chen, M.;# Cao, Z.;# Tang, W.; Wang, M.; Chen, Y.*; Guo, Z.*  Discovery of novel septacidin congeners from a high yield heterologous expression strain Streptomyces albus 1597. J Antibiot 2022, 75(3):172-175.

3. Guo, Z.; Tang, Y.; Tang, W.; Chen, Y.* Heptose-containing bacterial natural products: structures, bioactivities, and biosyntheses. Nat Prod Rep 2021, 38(10):1887-1909.

4. Li, C.; Bao, W., Zhang, H., Lyu, Z., Chen, Y., Guo, Z.*  Discovery of brominated alboflavusins with anti-MRSA activities. Front Microbiol 2021, 12, 641025.

5. Tang, W.; Chen, M.; Li, P.; Tang, Y.; Wang, M.; Guo, Z.*; Chen, Y.* Biosynthesis of the N⁶-glycosylated L-heptosamine-adenine core in septacidin. Org Lett 2020, 22 (13), 5251-5254.

6. Guo, Z. #; Li, P. #; Chen, G. #; Li, C.; Cao, Z.; Zhang, Y.; Ren, J.; Xiang, H.; Lin, S.; Ju, J.; Chen, Y*. Design and biosynthesis of dimeric alboflavusins with biaryl linkages via regiospecific C-C bond coupling. J Am Chem Soc 2018, 140, 18009-18015.

7. Tang, W#.; Guo, Z#.; Cao, Z.; Wang, M.; Li, P.; Meng, X.; Zhao, X.; Xie, Z.; Wang, W.; Zhou, A.; Lou, C.; Chen, Y*. D-Sedoheptulose-7-phosphate is a common precursor for the heptoses of septacidin and hygromycin B. Proc Natl Acad Sci USA, 2018, 115 (11), 2818-2823.

8. Guo, Z.#; Li, J.#; Qin, H. #; Wang, M.; Lv, X.; Li, X.; Chen, Y.* Biosynthesis of the carbamoylated D-gulosamine moiety of streptothricins: involvement of a guanidino-N-glycosyltransferase and an N-acetyl-D-gulosamine deacetylase. Angew Chem Int Ed 2015, 54 (17), 5175-5178.

9. Guo, Z.*; Shen, L.; Ji, Z.; Wu, W.*, Enhanced production of a novel cyclic hexapeptide antibiotic (NW-G01) by Streptomyces alboflavus 313 using response surface methodology. Int J Mol Sci, 2012, 13 (4), 5230-5241.

10. Guo, Z.; Shen, L.; Zhang, J.; Xin, H.; Liu, W.; Ji, Z.; Wu, W., NW-G03, a related cyclic hexapeptide compound of NW-G01, produced by Streptomyces alboflavus 313. J Antibiot 2011, 64 (12), 789-794.

11. Guo, Z.; Ji, Z.; Zhang, J.; Deng, J.; Shen, L.; Liu, W.; Wu, W., NW-G01, a novel cyclic hexapeptide antibiotic, produced by Streptomyces alboflavus 313: II. Structural elucidation. J Antibiot 2010, 63 (5), 231-235.

12. Guo, Z.; Shen, L.; Ji, Z.; Zhang, J.; Huang, L.; and Wu, W. NW-G01, a novel cyclic hexadepsipeptide antibiotic, produced by Streptomyces alboflavus 313: I. Taxonomy, fermentation, isolation, physicochemical properties and antibacterial activities. J Antibiot 2009, 62, 201-205.

13. Li, P.; Chen, M.; Tang, W.; Guo, Z.; Zhang, Y.; Wang, M.; Horsman, G. P.; Zhong, J.; Lu, Z.; Chen, Y., Initiating polyketide biosynthesis by on-line methyl esterification. Nat Commun 2021, 12 (1), 4499.

14. Wang, M.; Xie, Z.; Tang, S.; Chang, E. L.; Tang, Y.; Guo, Z.; Cui, Y.; Wu, B.; Ye, T.; Chen, Y.* Reductase of Mutanobactin synthetase triggers sequential C-C macrocyclization, C-S bond formation, and C-C bond cleavage. Org Lett 2020, 22 (3), 960-964.

15. Tang, Y.; Wang, M.; Qin, H.; An, X.; Guo, Z.; Zhu, G.; Zhang, L.; Chen, Y.* Deciphering the biosynthesis of TDP-beta-L-oleandrose in Avermectin. J Nat Prod 2020, 83 (10), 3199-3206.

16. Hao, T.; Xie, Z.; Wang, M.; Liu, L.; Zhang, Y.; Wang, W.; Zhang, Z.; Zhao, X.; Li, P.; Guo, Z.; Gao, S.; Lou, C.; Zhang, G.; Merritt, J.; Horsman, G. P.; Chen, Y.* An anaerobic bacterium host system for heterologous expression of natural product biosynthetic gene clusters. Nat Commun 2019, 10 (1), 3665.

17. Gao, C.; Guo, Z.; Lu, X.; Chen, H.; Liu, L.; Yu, Z.*; Chen, Y.* Hexaricins, Pradimicin-like polyketides from a marine sediment-derived Streptosporangium sp. and their antioxidant effects. J Nat Prod 2018, 81 (9), 2069-2074.

18. Li, P.; Guo, Z.; Tang, W.; Chen, Y.* Activation of three natural product biosynthetic gene clusters from Streptomyces lavendulae CGMCC 4.1386 by a reporter-guided strategy. Synth Syst Biotechnol 2018, 3 (4), 254-260.

19. Tian, J.; Chen, H.; Guo, Z.; Liu, N.; Li, J.; Huang, Y.; Xiang, W.; Chen, Y.* Discovery of pentangular polyphenols hexaricins A-C from marine Streptosporangium sp. CGMCC 4.7309 by genome mining. Appl Microbiol Biotechnol 2016, 100 (9), 4189-4199.

20. Li, P.; Li, J.; Guo, Z.; Tang, W.; Han, J.; Meng, X.; Hao, T.; Zhu, Y.; Zhang, L.; Chen, Y.* An efficient blue-white screening based gene inactivation system for Streptomyces. Appl Microbiol Biotechnol 2015, 99 (4), 1923-33.

21. Luo, X.; Cao, J.; Huang, J.; Wang, Z.; Guo, Z.; Chen, Y.; Ma, S.; Liu, J.* Genome sequencing and comparative genomics reveal the potential pathogenic mechanism of Cercospora sojina Hara on soybean. DNA research, 2017.

22. Fan, L.; Ji, Z.; Guo, Z.*; Wu, W.* NW-G12, A novel nonchlorinated cyclohexapeptide from Streptomyces alboflavus 313. Chem Nat Compd 2013, 49, 910-914.

Li, S.; Ji, Z.; Zhang, J.; Guo, Z.; Wu, W.* Synthesis of 1-acyl-3-isopropenylbenzimida-zolone derivatives and their activity against Botrytis cinerea. J Agric Food Chem 2010, 58, 2668-2672.

Awards and Honors :

2017  the Youth Innovation Promotion Association of the Chinese Academy of Sciences.