中国医学科学院医药生物技术研究所

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ANTI-INFLAMMATORY EFFECT OF EBOSIN ON RAT COLLAGEN-INDUCED ARTHRITIS THROUGH SUPPRESSING PRODUCTION OF INTERLEUKIN-1β, INTERLEUKIN-6 AND TUMOR NECROSIS FACTOR-α
来源: 时间:2014-07-07 浏览次数:2043
EUROPEAN JOURNAL OF INFLAMMATION   Vol. 11, no. 3, 677-688 (2013)
 
ANTI-INFLAMMATORY EFFECT OF EBOSIN ON RAT COLLAGEN-INDUCED ARTHRITIS THROUGH SUPPRESSING PRODUCTION OF INTERLEUKIN-1β, INTERLEUKIN-6 AND TUMOR NECROSIS FACTOR-α
Y. ZHANG1, L.F. WANG1, J.Y. BAI2, M.H. GUAN2, R. JIANG1,
L.H. GUO1, J.B. WU1, R. ZHANG3, G.F. CHENG2 and Y. LI1
Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; 2Institute of Material Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; 3School of Biological Sciences, University of Wollongong, NSW, Australia

1
Received May16, 2013 – Accepted July 29, 2013

 
Reumatoid arthritis (RA) is an autoimmune disease which has been studied experimentally using a wide variety of animal models including collagen-induced arthritis (CIA). Using this CIA model we studied the therapeutic effects and mechanism of action of Ebosin, a novel exopolysaccharide produced by Streptomyces sp. 139, on arthritis. Ebosin at 200, 400 and 600 mg/kg/day was orally administered to rats respectively between day 10 and 30 after immunization with chicken type II collagen. With the treatment arthritic progression was remarkably suppressed. Levels of anti-type II collagen–specific antibody, IL-1β and TNF-α were significantly lower in the Ebosin-treated CIA rats compared with the untreated controls. In cultured fibroblast-like synoviocytes (FLS), remarkable suppression of IL-1β, TNF-α and IL-6 production was detected at both protein and mRNA levels after Ebosin administration. Ebosin also resulted in lower activities of IL-1β-converting enzyme and TNF-α-converting enzyme in FLS. Based on these results, it is concluded that development and progression of rat CIA can be significantly suppressed by orally-administrated Ebosin. The therapeutic effect may be attributed to its inhibition in the production of IL-1β, TNF-a and IL-6 in the CIA rats.